Journal: Animals : an Open Access Journal from MDPI

Article Title: Microvascularization and Expression of Fibroblast Growth Factor and Vascular Endothelial Growth Factor and Their Receptors in the Mare Oviduct

doi: 10.3390/ani11041099

Figure Lengend Snippet: Primer sequences used for gene transcription analysis by real-time PCR.

Article Snippet: The relative protein abundance of FGFR1, FGFR2 and KDR was measured using specific primary antibodies against FGFR1 (Orb 156864, Biorbyt, Cambridge, UK, dilution 1:500), FGFR2 (SC 6930, Santa Cruz Biotechnology, Dallas, USA, dilution 1:500) and KDR (Orb 99143, Biorbyt, Cambridge, UK, dilution 1:250).

Techniques: Sequencing, Amplification

Journal: Animals : an Open Access Journal from MDPI

Article Title: Microvascularization and Expression of Fibroblast Growth Factor and Vascular Endothelial Growth Factor and Their Receptors in the Mare Oviduct

doi: 10.3390/ani11041099

Figure Lengend Snippet: Relative quantification of FGFR1 , FGFR2 and KDR transcripts in the equine oviduct ( n = 5 samples for each estrous cycle phase; n = 5 for each portion of oviduct analyzed). Comparison of transcripts between the follicular phase, early-luteal phase (Early LP), and mid-luteal (Mid LP) ( A , C , E ). Comparison of transcripts between oviduct portions (infundibulum, ampulla, isthmus), regardless of the phase of the estrous cycle ( B , D , F ). Bars represent mean ± SEM. AU: arbitrary units. Different letters indicate significant differences ( p < 0.05).

Article Snippet: The relative protein abundance of FGFR1, FGFR2 and KDR was measured using specific primary antibodies against FGFR1 (Orb 156864, Biorbyt, Cambridge, UK, dilution 1:500), FGFR2 (SC 6930, Santa Cruz Biotechnology, Dallas, USA, dilution 1:500) and KDR (Orb 99143, Biorbyt, Cambridge, UK, dilution 1:250).

Techniques:

Journal: Animals : an Open Access Journal from MDPI

Article Title: Microvascularization and Expression of Fibroblast Growth Factor and Vascular Endothelial Growth Factor and Their Receptors in the Mare Oviduct

doi: 10.3390/ani11041099

Figure Lengend Snippet: Relative protein abundance of FGFR1, FGFR2 and KDR in the equine oviduct ( n = 5 samples for each estrous cycle phase; n = 5 for each portion of oviduct analyzed). Comparison of relative protein abundance between oviduct portions (infundibulum, ampulla, isthmus), regardless of the phase of the estrous cycle ( A , C , D ), and their respective representative Western blot bands ( B , D , F ). Bars represent mean ± SEM. AU: arbitrary units. Different letters indicate significant differences ( p < 0.05).

Article Snippet: The relative protein abundance of FGFR1, FGFR2 and KDR was measured using specific primary antibodies against FGFR1 (Orb 156864, Biorbyt, Cambridge, UK, dilution 1:500), FGFR2 (SC 6930, Santa Cruz Biotechnology, Dallas, USA, dilution 1:500) and KDR (Orb 99143, Biorbyt, Cambridge, UK, dilution 1:250).

Techniques: Western Blot

Journal: Scientific Reports

Article Title: A novel signaling pathway regulates colon cancer angiogenesis through Norrin

doi: 10.1038/srep05630

Figure Lengend Snippet: In column 1, low power (5×) micrographs of the tumor microenvironment are depicted. The square demonstrates the region of higher (100×) magnification (columns 2–5). Green fluorescence (column 2) indicates expression of a specific Norrin signaling component (see row designation, below). Red fluorescence (column 3) marks endothelial cells (anti-CD31 positive) of colorectal tumor blood vessels. Blue fluorescence(column 4) depicts DAPI-stained nuclei. Merged images are depicted in column 5. Row (A). Expression of Norrin in colorectal tumor endothelial cells. Row (B). Expression of Frizzled 4 in colorectal tumor endothelial cells. Row (C). Expression of Lrp5 in colorectal tumor endothelial cells. Row (D). Expression of TSPAN12 in colorectal tumor endothelial cells.

Article Snippet: Colorectal tumor slides were triple stained with DAPI (blue) for nuclear DNA, Texas Red (red) for human blood vessel endothelial cells (anti-CD31 mouse monoclonal antibody Abcam (ab 9498) at 1:10 dilution, and a secondary antibody conjugate Texas Red-donkey polyclonal against mouse IgG from Abcam (ab 7059) used at 1:1000 dilution) and FITC (green) for components of Norrin pathway (goat polyclonal directed against human Norrin (R&D, AF 3014), diluted till 1:50 from stock 0.2 mg/ml; rabbit polyclonal against human Frizzled 4 (MBL International Corporation, MC-4911), diluted 1:40; goat polyclonal against human Lrp 5 (Abcam, ab 36121), diluted 1:500; rabbit polyclonal against human TSPAN12 (Biorbyt, orb 39384), diluted at 1:100.

Techniques: Fluorescence, Expressing, Staining